Immunization of broiler chicks by in ovo injection of Eimeria tenella sporozoites, sporocysts, or oocysts

Effects of Different Sizes of Glass Beads on the Release of Sporocysts from Eimeria tenella Oocysts

The oocyst wall is severed by means of mechanical injury or chemical agents. This study reports the percentage of in vitro sporocyst release following mechanical shaking in the presence of varying sizes of glass beads. Glass beads measured 0.5, 1, and 3 mm in diameter and were shaken with the oocysts for different times ranging from 5 sec to 5 min. Approximately 80% of sporocysts were released ...

Enhanced egress of intracellular Eimeria tenella sporozoites by splenic lymphocytes

Studies of a glycoprotein in the oocysts of Eimeria tenella.

A glycoprotein unique to the cytoplasm of the unsporulated oocyst of Eimeria tenella has been purified and partially characterized. The protein has a molecular weight of 30,000, of which approximately 40% is carbohydrate. The carbohydrate portion of the molecule consists of glucose, galactose, mannose, xylose, glucosamine, and galactosamine, with no detectable sialic acid. The protein portion c...

Immunization of young chicks using graded dose of wild strain of Eimeria tenella.

A wild strain of Eimeria tenella was isolated and utilized for immunization studies. Its optimal sporulation was attained at room temperature 24-25 degrees C after 24-48 h. Two groups of chicks were immunized by dosing a graded dose of five oocysts/chick/day for 6 days followed by 50 oocysts/chick/day for 7 days. A third group was not immunized and served as a negative control. Immunized chicks...

INTRATRACHEAL INFECTION OF CmCKHNS WITH SPORULATED OOCYSTS OF EIMERIA TENELLA

Sanda A., Salman Al Said: Intratracheal Infection of Chickens with Sporulated Oocysts of Eimeria tenella. Acta vet. Bmo, 47, 1978: 185-187. Experiment involving 16 treated and 10 control chickens, aged 3 weeks, was conducted to study the intratracheal route of infection by sporulated oocysts of Eimeria tenella. In the experimental birds a surgical procedure was conducted to prevent ingestion of...